Atdc5 细胞分化软骨诱导方法
WebATDC5 mouse chondroprogenitor cells were purchased commercially (Sigma Aldrich, St. Louis, MO). ATDC5 mouse chondroprogenitor cells were used to conduct all experiments. For all experiments, cells were cultured in media consisting of a 1:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F12 containing 5% fetal bovine serum, 100 Webプは,こ の実験系を用いて,atdc5の 軟骨細胞への分化 について検討した結果,イ ンスリン存在下で培養すると, 生体内で見られる軟骨の分化段階のすべて(図2)を シュ ミレートできることを示した(4).こ の実験系で軟骨細胞
Atdc5 细胞分化软骨诱导方法
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WebAfter ATDC5 cells were induced by IL-1β, the expression of SREBF2 in osteoarthritis cells was detected by RT-qPCR. The content of iron ion in the cells was detected by using an iron colorimetric assay kit. After further transfection of a SREBF2 overexpressing vector (Oe-SREBF2) or addition of a ferroptosis inhibitor, the expression levels of ... WebATDC5 cells were transfected with AKT2 or treated with metformin (0.5, 1, 2, and 4 mM) and incubated for 2 (A–D), 4 (E–H), 6 (I–L), 12 (M–P) hours. At the end of the experiment, cell lysates were collected and Western blot analysis was performed (n = 3). Metformin significantly inhibits β-catenin S552 phosphorylation, cyclin D1 and ...
WebNov 28, 2012 · The ATDC5 cell line is derived from mouse teratocarcinoma cells and characterized as a chondrogenic cell line which goes through a sequential process analogy to chondrocyte differentiation. Thus, it is regarded as a promising in vitro model to study the factors that influence cell behaviors during chondrogenesis. It also provides insights in ... WebATDC5细胞系增殖分化各阶段标志物表达差异的比较. 【摘要】： 软骨损伤是一种临床常见病,包括磨损与老年后发生的退行性病变,由于软骨内缺乏血管、神经等组织,导致软骨一 …
WebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat … WebDec 27, 2016 · CHICAGO — If you think your neighborhood has changed since you first moved in, you should see what it looked like 60 years ago. The University of Illinois at …
WebNov 2, 2024 · このatdc5はbmpシグナル等の働きで軟骨分化が亢進するとalp 注5) がたくさん産生されるため、このalpの働きを異所性骨化につながる異常な軟骨分化の指標として測定するハイスループットスクリーニング系を構築しました。
WebThe ATDC5 cell line exhibits a multistep process of chondrogenic differentiation analogous to that observed during endochondral bone formation. Previous investigators have induced ATDC5 cells to differentiate by exposing them to insulin at high concentrations. We have observed spontaneous differentiation of ATDC5 cells maintained in ascorbic ... mercedes b class edinburghWebATDC5是一种重要的软骨细胞模型,已经被广泛地应用于软骨细胞增殖分化等方面的研究,但关于ATDC5细胞在各增殖与分化阶段标志物如何变化方面的研究较少。. 因此,为了阐 … how often should a man ejaulatehttp://manu45.magtech.com.cn/Jwk_kqyxyj/CN/10.13701/j.cnki.kqyxyj.2024.05.014 how often should a male urnateWebDownload scientific diagram ATDC5 cell differentiation in culture. To assess induction of cell proliferation and differentiation, cells were cultured in 12-well plates at a density of 50,000 ... how often should a man ejaulate to be healthyWebAug 31, 2012 · For the latter, ATDC5 cells were chosen as an optimized model for rapid and physiological matrix maturation [43, 44]. Using the immunofluorescence imaging of NFAT5/NFAT5, we first showed a similar ... mercedes b class motabilityWebChicago Tribune obituaries and Death Notices for Chicago Illinois area . Explore Life Stories, Offer Condolences & Send Flowers. mercedes b class parkersWebSep 25, 2024 · It is known that Wnt/β-catenin signaling induces endochondral ossification and plays a significant role in the pathophysiology of osteoarthritis (OA). Sclerostin is a potent inhibitor of the Wnt/β-catenin signaling pathway. This study investigated the role of sclerostin in the endochondral differentiation under an OA-like condition induced by … mercedes b class length