Easytaq buffer
Web- TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. - PCR products can be directly cloned into pEASY®-Blunt vectors. ... Buffer Storage Buffer: 50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol 5xTransStart® FastPfu Buffer with 20 mM MgSO4: 100 mM Tris-SO4 ( pH … WebAug 31, 2024 · The reaction system was prepared using the EasyTaq® DNA Polymerase kit (TransGen Biotech, Beijing, China): 1 μl EasyTaq® DNA Polymerase, 5 μl template, 2 μl 10× EasyTaq® Buffer, 2 μl 2.5 mM dNTPs, 2 μl primer mix, 8 μl ddH 2 O. Each 100 μl of primer mix contains 1 μl of primers of all the 33 targets bacterium, IC, and human DNA ...
Easytaq buffer
Did you know?
WebSep 25, 2024 · PCR was carried out in 50 μL reactions, including the template DNA (10 ng), 5 μL of 10 × EasyTaq buffer, 2 μL of 2.5 mM dNTPs, 1 μL of EasyTaq DNA polymerase (5 units), 1 μL of each primer (10 μM), and nuclease-free water. The PCR conditions were as follows: 95 °C for 2 min, 30 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for ... WebMar 21, 2024 · The error-prone PCR reaction mixture contained 10 × EasyTaq buffer (2.5 μL), ddH 2 O (16.0 μL), dNTPs (2.5 mM, 2 μL), Mn 2+ (1 mM, 2.5 μL), template plasmid (0.5 μL), forward and reverse primers (10 μM, 0.5 μL each), and EasyTaq DNA polymerase (2.5 U). The PCR program was 3 min denaturation at 94 ºC, followed by 30 cycles of 95 ºC for ...
WebDec 15, 2024 · downstream primers, 1 µl of EasyTaq® DNA polymerase, and 20 µl of P-mother liquor (P-mother liquor was prepared by mixing the following reagents: 10× EasyTaq® buffer:2.5 mM dNTPs:H 2O=5:3:32). The reaction procedure was as follows: 94 °C for 3 min, 35 cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C WebEasyTaq® DNA Polymerase is isolated from E. coli expressing a cloned DNA polymerase gene from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. ... Storage Buffer. 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. Storage-20°C for two years ...
WebTaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.. For … WebThe 10x EasyTaq buffer is used with EasyTaq DNA Polymerase. Additional information. Supplier: Transgen Biotech. Format: 1.2 ml. Ask a question about 10x EasyTaq Buffer (with Mg2+) – GK101. Name. Email Address. Competitor product (if applicable) Question. Get an answer quickly.
Web10x EasyTaq Buffer (with Mg2+) – GK101. Only 60$ when you add 5 vials (6 ml total volume). $ 14.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. Only 60$ when …
WebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq ® DNA Polymerase. • Extension rate is about 1-2 kb/min. • Template-independent "A" can be generated at the 3' end of the ... blockguss stahlhttp://www.life-tech.org/2xeasytaq-pcr-supermix/ free bullying worksheetsWebAug 19, 2024 · The 20 μl PCR reaction mixture contained 10× EasyTaq buffer (2 μL), 2.5 mM dNTPs (1.6 μl), 5 U/μl EasyTaq DNA Polymerase (0.2 μl), 20 μM primers (1 μL each), crude DNA template (1 μl), and sterile distilled water. Amplification was performed on a thermal cycler with a cycling profile of initial denaturation at 95°C for 5 min; 30 ... free bully pitbull puppiesWebEasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2xconcentration and used at 1x concentration by adding template, primers and H2O. PCR products are not suitable for PAGE. Components. 2xEasyTaq® PCR SuperMix ( +dye ), ddH2O. blockgurg autofarm no band scriptWeb2x EasyTaq PCR SuperMix (+dye) The 2x EasyTaq PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer.The supermix is provided at 2x concentration and used at 1x concentration by adding template, primers and H 2 O. PCR products are unsuitable for PAGE. block gun warWebEasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous … block gun three d. call of destinyWebThermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also … free bumble bee clip art